兼任老師

  1. 兼任老師
林立涵 助理教授

林立涵 助理教授

林立涵 _2_.jpg
/
詳細介紹
電子郵件：volkdeskkimo@gmail.com
最高學歷：國立陽明大學口腔生物研究所博士 
專長：腫瘤生物學、基因體學
 

。學經歷

2016/02-迄今            馬偕醫療財團法人馬偕紀念醫院 助研究員
2017/08-2018/01     經國管理暨健康學院 助理教授
2014/08-2015/07     國立陽明交通大學 牙醫學院 博士級研究助理

。實驗室研究方向

        本實驗室的研究方在於探討口腔癌發展過程中基因體與轉錄體變化對癌症生物學的影響。口腔癌的形成涉及多個基因突變與表現量異常，而不同病患間的基因突變譜則呈現高度異質性。本實驗室使用臨床腫瘤檢體進行基因體定序實驗，以深入了解口腔癌的分子致癌機制，期望找出潛在的治療標靶，並提供個人化治療策略之依據。
目前我們已建立臨床檢體資料庫，並完成全外顯子定序（Whole-Exome Sequencing, WES）及轉錄體定序（RNA-seq）分析。WES結果顯示，TP53為口腔癌中最常發生突變的基因，且不同病人之間存在顯著的基因突變譜異質性（圖一）。RNA-seq分析顯示，約有2,600個基因在腫瘤組織與正常上皮組織間呈現顯著表現差異（圖二）。進一步整合WES與RNA-seq資料，我們近期發現 腫瘤中PFN2基因因基因增幅（gene amplification）導致大量表現，PFN2透過調控HDAC1活性促進腫瘤細胞進展 (圖三)。臨床存活分析顯示，PFN2高表現患者具有較差的預後，顯示其可能成為新的治療標靶。實驗室未來會整合WES和RNAseq數據，系統性解析腫瘤進展過程中的關鍵分子事件，期望建立可預測病程與治療反應的分子分類模型。
。近五年代表著作
  1. Hao-Wen Chuang, Li-Han Lin, Dar-Der Ji, Ting-Ying Fu, Herng-Sheng Lee, Yi-FangYang, Hsing-Cheng Tseng, Kan-Tai Hsia. Serpin peptidase inhibitor, clade E, member 2 is associated with malignant progression and clinical prognosis in oral squamous cell carcinoma. J Dent Sci. 2024 Jan;19(1):70-78.
  2. Li-Han Lin, Kuo-Wei Chang, Hui-Wen Cheng and Chung-Ji Liu. Identification of Somatic Mutations in Plasma Cell-Free DNA from Patients with Metastatic Oral Squamous Cell Carcinoma. Int J Mol Sci. 2023 Jun 20;24(12):1. 
  3. Hsuan yu Chen, Li‑Han Lin and Chung Ji Liu. Risk factors for reconstruction plate exposure after surgery in oral cancer patients. A retrospective cohort study. Clin Oral Investig 2022 May;26(5):4127-4136.
  4. Li-Han Lin, Hui-Wen Cheng and Chung-Ji Liu. Droplet digital polymerase chain reaction for detection and quantification of cell-free DNA TP53 target somatic mutations in oral cancer. Cancer Biomark. 2022;33(1):29-41.
  5. Hsi-Feng Tu, Li-Han Lin, Kuo-Wei Chang, Hui-Wen Cheng, Chung-Ji Liu. Exploiting salivary miR-375 as a clinical biomarker of oral potentially malignant disorder. J Dent Sci. 2022 Apr;17(2):659-665.
  6. Li-Han Lin, Chung-Hsien Chou, Hui-Wen Cheng, Kuo-Wei Chang and Chung-Ji Liu. Precise Identification of Recurrent Somatic Mutations in Oral Cancer Through Whole-Exome Sequencing. Front Oncol. 2021 Nov 29:11:741626.
  7. Li-Han Lin, Jiun-Sheng Lin, Cheng-Chieh Yang, Hui-Wen Cheng, Kuo-Wei Chang, and Chung-Ji Liu. Overexpression of Platelet-Derived Growth Factor and Its Receptor Are Correlated with Oral Tumorigenesis and Poor Prognosis in Oral Squamous Cell Carcinoma. Int J Mol Sci. 2020 Mar 29;21(7):2360.
  8. Li-Han Lin, Kuo-Wei Chang, Hui-Wen Cheng and Chung-Ji Liu. SMAD4 Somatic Mutations in Head and Neck Carcinoma Are Associated With Tumor Progression. Front Oncol. 2019 Dec 6:9:1379.
  9. Yi-Fen Chen, Chung-Ji Liu, Li-Han Lin, Chung-Hsien Chou, Li-Yin Yeh, Shu-Chun Lin and Kuo-Wei Chang. Establishing of mouse oral carcinoma cell lines derived from transgenic mice and their use as syngeneic tumorigenesis models. BMC Cancer. 2019 Mar 29;19(1):281.
  10. Li-Han Lin, Kuo-Wei Chang, Shou-Yen Kao, Hui-Wen Cheng and Chung-Ji Liu. Increased Plasma Circulating Cell-Free DNA Could Be a Potential Marker for Oral Cancer. Int J Mol Sci. 2018 Oct 24;19(11):3303.
  11. Shu-Chun Lin, Li-Han Lin, Ssu-Yu Yu, Shou-Yen Kao, Kuo-Wei Chang, Hui-Wen Cheng and Chung-Ji Liu. FAT1 somatic mutations in head and neck carcinoma are associated with tumor progression and survival. Carcinogenesis. 2018 Dec 13;39(11):1320-1330.
  12. Ming-Fang Cheng, Li-Han Lin, Mao-Suan Huang, Herng-Sheng Lee, Dar-Der Ji, Chun-Shu Lin and Kan-Tai Hsia. Downexpression of Matriptase-2 Correlates With Tumor Progression and Clinical Prognosis in Oral Squamous-Cell Carcinoma. Appl Immunohistochem Mol Morphol. 2017 Aug;25(7):481-488.
  13. Kan-Tai Hsia, Chung-Ji Liu, Kwei Mar, Li-Han Lin, Chun-Shu Lin, Ming-Fang Cheng, Herng-Sheng Lee and Shang-Yi Chiu. Impact of apurinic/apyrimidinic endonuclease 1/redox factor-1 on treatment response and survival in oral squamous cell carcinoma. Head Neck. 2016 Apr;38(4):550-9.
  14. Li-Han Lin, Ming-Wei Lin, Kwei Mar, Chun-Shu Lin, Dar-Der Ji, Wei-Ping Lee, Herng-Sheng Lee, Ming-Fang Cheng and Kan-Tai Hsia. The hMLH1 -93G>A promoter polymorphism is associates with outcomes in oral squamous cell carcinoma patients. Ann Surg Oncol. 2014 Dec;21(13):4270-7.
  15. Ming-Fang Cheng, Mao-Suan Huang, Chun-Shu Lin, Li-Han Lin, Herng-Sheng Lee, Jhih-Cheng Jiang and Kan-Tai Hsia. Expression of matriptase correlates with tumour progression and clinical prognosis in oral squamous cell carcinoma. Histopathology. 2014 Jul;65(1):24-34. 
回上頁